A rate - limiting step to long - term survival in heart transplant patients is coronary arteriopathy , which is marked intimal proliferation with lumenal narrowing of the small epicardial and intramyocardial coronary artery branches , leading to ischemic changes 冠狀動脈影響到心臟移植病人能否長期存活,它的特征有內(nèi)膜增生伴有心外膜以及心肌內(nèi)的冠狀動脈分支管腔狹窄,從而導(dǎo)致缺血性改變。
Translocation from endocytic compartments to the cytosol is the essential and rate - limiting step in the intoxication process of most toxins such as ricin , diphtheria toxin , shiga toxin and pseudomonas exotoxin ( pe ) . a number of these toxins are transported to trans - golgi network ( tgn ) , and in many cases such transport to the tgn is required for the translocation and cytotoxicity . in deed , 5 % of the ricin endocytosed by cells has been shown to reach the tgn 蓖麻毒素進入細胞的機理不甚明了,一般認(rèn)為是rtb先與細胞膜受體結(jié)合,主要經(jīng)過受體介導(dǎo)的內(nèi)吞作用進入吞噬體,然后沿著內(nèi)體、高爾基體、內(nèi)質(zhì)網(wǎng)等逆向分泌途徑,有序地運輸?shù)絻?nèi)質(zhì)網(wǎng),最后從內(nèi)質(zhì)網(wǎng)轉(zhuǎn)位進入細胞漿,在胞漿內(nèi)攻擊核糖體,從而抑制蛋白質(zhì)的合成,導(dǎo)致細胞死亡。
In order to break down the rate - limited steps in the artemisinin biosynthesis to improve the artemisinin production and realize the industrial production of artemisinin , related key genes in artemisinin biosynthesis must be cloned and the regulatory patterns of key genes should be studied . for this purpose molecular cloning of related key genes in artemisinin biosynthesis was performed in this thesis work 利用現(xiàn)代分子生物學(xué)和基因工程技術(shù)手段,克隆青蒿素生成途徑的關(guān)鍵酶基因,研究關(guān)鍵酶基因?qū)η噍锼厣锖铣傻恼{(diào)控規(guī)律,是打破青蒿素生物合成的限速步驟,大幅度提高青蒿素含量,最終達到利用植物生物技術(shù)工業(yè)化生產(chǎn)青蒿素的目的必須解決的關(guān)鍵問題。